LysetTM is manufactured by Sclavo Diagnostics International S.r.l. on Biorigen S.r.l. licence

BOOST YOUR CELL CULTURE TODAY FOR THE EXPERIMENTS OF TOMORROW

an “off the shelf”, fully standardized and lyophilized, human platelet derived cell culture supplement.

XENO-FREE COMPONENTS OF HUMAN ORIGIN

is being utilized to replace animal and human serum such as Fetal Bovine Serum (FBS) in culture medium formulation for different types of cells.

CELL SUPPLEMENT OBTAINED BY THE COMBINATION OF TWO COMPONENTS

the possibility of mixing the two supplement components (PL, human platelet lysate from Platelet Rich Plasma and AD, human Platelet Poor Plasma) in different percentages, allows the supplement customization and optimization for each cell type.

References

References on Platelet Lysate for Cell Culture

1-El Backly R, Ulivi V, Tonachini L, Cancedda R, Descalzi F and Mastrogiacomo M. Platelet Lysate Induces In Vitro Wound Healing of Human Keratinocytes Associated with a Strong Proinflammatory Response; Tissue Engineering: Part A Volume 17, (13 and 14): 1787-1800, 2011.

2-Zaky SH, Ottonello A, Strada P, Cancedda R and Mastrogiacomo M. Platelet lysate favours in vitro expansion of human bone marrow stromal cells for bone and cartilage engineering. J Tissue Eng Regen Med; 2: 472-481, 2008.

1-Additional references

1-Avanzini MA, Bernardo ME,Cometa AM, Perotti C, Zaffaroni N, Novara F, Visai L, Del Fante C, Ball LM. Generation of mesenchymal stromal cells in the presence of platelet lysate: a phenotypic and functional comparison of umbilical cord-blood and bone marrow-derived progenitors. Haematologica; 94 (12):1649-1660, 2009.

2-Bernardo ME, Avanzini MA , Perotti C, et al. Optimization of in vitro expansion of human multipotent mesenchymal stromal cells for cell-therapy approaches: further insights in the search for a fetal calf serum substitute. J Cell Physiol; 211:121–130, 2007.

4-Bieback K, Hecker A, Kocaömer A, Lannert H, Schalmoser K, Strunk D, Klüter H, Human Alternatives to Fetal Bovine Serum for the Expansion of Mesenchymal Stromal Cells from Bone Marrow. Stem Cells; 27:2331–2341, 2009.

5-Blande IS, Bassaneze V, Lavini-Ramos C, Fae CC, Kalil J, Miyakawa AA, Schettert IT and KriegerJE. Adipose tissue mesenchymal stem cell expansion in animal serum-free medium supplemented with autologous human platelet lysate. Transfusion; 49: 2680-2685, 2009.

6-Brunner D, Frank J, Appl H et al. Serum-free cell culture: the serum-free media interactive online database. ALTEX; 27, 53-62, 2010.

7-Capelli C, Domenghini M, Borleri G, Bellavita P, Poma R, Carobbio A, Mico C, Rambaldi A, Golay J and Introna M. Human platelet lysate allows expansion and clinical grade production of mesenchymal stromal cells from small samples of bone marrow aspirates or marrow filter washouts. Bone Marrow Transplantation; 40:785–791, 2007.

8-Carrancio S, Lopez-Holgado C, Sanchez-Guijo FM, Villarona E, Barbadoa V, Tabera S, Diez-Campelo M, Blanco J, San Miguel JF andConsuelo del Canizo M, Experimental. Optimization of mesenchymal stem cell expansion procedures by cell separation and culture conditions modification. Hematology; 36:1014–1021, 2008.

9-Centeno CJ, Busse D, Kisiday J, Keohan C, Freeman M, Karli D. Regeneration of meniscus cartilage in a knee treated percutaneoulsy implanted autologous mesenchymal stem cells. Med Hypotheses; 71: 900-908, 2008.

10-Chan RK, Liu P, Lew DH et al..Expired liquid preserved platelet releasates retain proliferative activity. J Surg Res; 126, 55-58, 2005.

11-Chen B, Sun HH, Wang HG, Kong H, Chen FM, Yu Q. The effects of human platelet lysate on dental pulp stem cells derived from impacted human third molars. Biomaterials; in press Apr 17,2012

12.Coppinger JA, Cagney G, Toomey S et al. Characterization of the proteins released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions. Blood; 103: 2096–2104, 2004.

13-Crespo-Diaz R, Behfar A, Butler GW, Padley DJ, Sarr MG, Bartunek J, Dietz AB and Terzic A. Platelet Lysate Consisting of a Natural Repair Proteome Supports Human
Mesenchymal Stem Cell Proliferation and Chromosomal Stability. Cell Transplantation; Vol. 20, pp. 797–811, 2011

14-Dahl JA, Duggal S, Coulston N et al. Genetic and epigenetic instability of human bone marrow mesenchymal stem cells expanded in autologous serum or fetal bovine serum; Int J Dev Biol; 52:1033–1042, 2008.

15-Davenport M, Verrier S, Droeser R et al. Platelet lysate as a serum substitute for 2D static and 3D perfusion culture of stromal vascular fraction cells from human adipose tissue; Tissue Eng Part A; 15 :869–875, 2009.

16-Doucet C, Ernou I, Zhang Y, Llense JR, Begot L, Holy X, Lataillade JJ. Platelet lysates promote mesenchymal stem cell expansion: a safety substitute for animal serum in cell-based therapy applications. J Cell Physiol;205: 228-236, 2005.

17-ESAC (2008). Statement on the use of FCS and other animal- derived supplements. http://ecvam.jrc.it

18-Falkner E, Appl H, Eder C, Losert UM, Schöffl H and Pfaller W. Serum free cell culture: The free access online database. Toxicol In Vitro; 20: 395-400, 2006.

19-Fekete N, Gadelorge M, Fürst D, Maurer C, Dausend J, Fleury-Cappellesso S, Mailänder V, Lotfi R, Ignatius A, Sensebé L, Bourin P, Schrezenmeier H, Rojewski MT. Platelet lysate from whole blood-derived pooled platelet concentrates and apheresis-derived platelet concentrates for the isolation and expansion of human bone marrow mesenchymal stromal cells: production process, content and identification of active components. Cytotherapy; 14(5):540-54, 2012.

20-Friedenstein AJ, Deriglasova UF, Kulagina NN, Panasuk AF,Rudakowa SF, Luria` EA et al. Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method. Exp Hematol; 2: 83–92,1974.

21-Frou SJ. The development, benefits and disadvantages of serum-free media. Dev Biol Stand; 99, 157-166, 1999.

22-Giacco F, D’Agostino E, Fratellanza G, Perna E, Misso S, Saldalamacchia G, Oriente F, Fiory F, Miele C, Formisano S, Beguinot F and Formisano P. Thrombin-activated platelets induce proliferation of human skin fibroblast by stimulating autocine production of insulin-growth factor. FASEB J; 20:2402-2404, 2006.

23-Honn KV, Singley JA, Chavin W. Fetal bovine serum: A multivariate standard. Proc Soc Exp Biol Med; 149:344–347, 1975.

24-Italiano JE Jr, Richardson JL, Patel-Hett S et al. Angiogenesis is regulated by a novel mechanism: Pro- and antiangiogenic proteins are organized into separate platelet alpha granules and differentially released. Blood; 111:1227–1233, 2008.

25-Jayme DW, Epstein DA and Conrad DR. Fetal bovine serum alternatives.Nature; 334: 547-548, 1988.

26-Johansson, l., Klinth, J., Holmqvist, O., and Ohlson, S.. Platelet lysate: a replacement for fetal cell culture? Cytotechnol; 42: 67-74, 2003.

27-Jochems CEA, van der Valk JBF, Stafleu FR and Baumans V. The use of fetal bovine serum: ethical or scientific problem? ATLA; 30: 219-227, 2002. Kakudo N . Proliferation-promoting effect of platelet-rich plasma on human adipose-derived stem cells and human dermal fibroblasts. Plast Reconstr Surg; 122: 1352-1360, 2008.

28-Kaps C, Loch A, Haisch A et al.Human platelet supernatant promotes proliferation but not differentiation of articular chondrocytes. Med Biol Eng Comput; 40:485-490, 2002.

29-Kobayashi T, Watanabe H, Yanagawa T et al. Motility and growth of human bone-marrow mesenchymal stem cells during ex vivo expansion in autologous serum. J Bone Joint Surg Br;87:1426–1433, 2005.

30-Kocaömer A, Kern S, Kluter H, Bieback K. Human AB serum and thrombin-activated platelet-rich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue. Stem Cells; 25:1270-1278, 2005.

31-Lange C, Cakiroglu F, Spiess A, Cappalo-Obermann H, Zander A. Platelet lysate for rapid expansion of human mesenchymal stromal cells. Cell Ther Transplant; 1 (2): 49-53, 2008

32-Lin HT, Tarng YW, Chen YC et al. Using human plasma supplemented medium to cultivate human bone marrow-derived mesenchymal stem cell and evaluation of its multiple-lineage potential. Transplant Proc; 37:4504–4505, 2005.

33-Luttenberger T, Schmid-Kotsas A, Menke A, Siech M, Beger H, Adler G, Grünert A and Bachem MG. Platelet-Derived Growth Factors Stimulate Proliferation and Extracellular Matrix Synthesis of Pancreatic Stellate Cells: Implications in Pathogenesis of Pancreas Fibrosis. Lab Invest; 80 (1): 47-55,2000.

34-Mannello F, Tonti GA. Concise review: No breakthroughs for human mesenchymal and embryonic stem cell culture: conditioned medium, feeder layer, or feeder-free; medium with fetal calf serum, human serum, or enriched plasma; serum-free, serum replacement nonconditioned medium, or ad hoc formula? All that glitters is not gold! Stem Cells; 25:1603–1609, 2007.

35-Müller AM, Davenport M, Verrier S, Droeser R, Alini M, Bocelli-Tyndall , Schaefer DJ, Martin I and Scherberich A. Platelet Lysate as a Serum Substitute for 2D Static and 3D Perfusion Culture of Stromal Vascular Fraction Cells from Human Adipose Tissue .Tissue Engineering: Part A;15, 4 :869-875, 2009.

36-Müller I, Kordowich S, Holzwarth C et al. Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM. Cytotherapy; 8:437–444, 2006.

37-Ogino Y, Ayukawa Y, Kukita T and Koyano K. The contribution of platelet-derived growth factor, transforming growth factor-beta1, and insulin-like growth factor-I in plate- let-rich plasma to the proliferation of osteoblast-like cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod; 101, 724-729, 2006.

38-Prins HJ, RozemullerH, Vonk-Griffioen S, Verweij VGM, Dhert WJA,Slaper-Cortenbach ICM and Martens ACM. Bone-Forming Capacity of Mesenchymal Stromal Cells When Cultured in the Presence of Human Platelet Lysate as Substitute for Fetal Bovine Serum.Tissue Engineering Part A; 15, 12:3741-3759, 2009.

39-Rauch C, Feifel E, Schöffl H, Pfaller W, Gstraunthaler G. Alternatives to the use of fetal bovine serum: Platelet lysates as serum replacement in cell and tissue culture. ALTEX ; 24(4): 353, 2007.

40-Reichl H, Balen A, Jansen CA. Prion transmission in blood and urine: what are the implications for recombinant and urinary-derived gonadotrophins? Hum Reprod; 17:2501–2508, 2007.

41-Reinisch A, Rohde E, Schallmoser K,Bjelic-Radisic, Lanzer G, Linkesch W and Strunk D. Humanized system to propagate cord blood-derived multipotent mesenchymal stromal cells for clinical application. Regen Med; 2(4): 371-382, 2007.

42-Schallmoser K, Bartmann C, Rohde E, Reinisch A, Kashofer K, Stadelmeyer E, Drexler C, Lanzer G, Linkesch W, Strunk D. Human platelet lysate can replace fetal bovine serum for clinical-scale expansion of functional mesenchymal stromal cells. Transfusion; 47:1436-1446, 2007.

43-Schallmoser K, Rohde E, Reinisch A et al. Rapid large-scale expansion of functional mesenchymal stem cells from unmanipulated bone marrow without animal serum. Tissue Eng Part C Methods; 14: 185–196, 2008.

44-Schallmoser K, Strunk D. Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal-Free Human Stem Cell Cultures. J Vis Exp, 32: 1-10,2009.

45-Shahdadfar A, Fronsdal K, Haug T, Reinholt FP, Brinchmann JE. In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression, and transcriptome stability. Stem Cells; 23: 1357-1366, 2005.

46-Spreafico A, Chellini F, Frediani B et al.. Biochemical investigation of the effects of human platelet releasates on human articular chondrocytes. J. Cell. Biochem; 108, 1153-1165, 2009.

47-Stute N, Holtz K, Bubenheim M, Lange C, Blake F, Zander AR. Autologous serum for isolation and expansion of human mesenchymal stem cells for clinical use. Exp Hematol; 32:1212-1225, 2004.

48-Umeno Y, Okuda A, Kimura G, Proliferative behavior of fibroblasts in plasma –rich culture medium. J Cell Sc ; 94 : 567-575, 1989.

49-van der Valk J, Mellor D, Brands R, Fischer R, Gruber F, Gstraunthaler G, Hellebrekers L, Hyllner J, Jonker FH, Prieto P, Thalen M and Baumans V. The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture. Toxicol. In Vitro; 18: 1-12, 2004.

50-Von Bonin M, Stolzel F, Goedecke A et al. Treatment of refractory acute GVHD with third-party MSC expanded in platelet lysate-containing medium. Bone Marrow Transplant; 43:245–251, 2009.

51-Wessman SJ and Levings RL. Benefits and risks due to animal serum used in cell culture pro- duction. Dev Biol Stand; 99, 3-8, 1999.

2-References on Growth Factors in Anti-Wrinkles Therapy:

1-Bhanot S, Alex JC. Current applications of platelet gels in facial plastic surgery. Facial Plast Surg. 2002; 18(1):27-33, 2002.

2-Cho JM, Lee YH, Baek RM, Lee SW. Effect of platelet-rich plasma on ultraviolet b-induced skin wrinkles in nude mice. JPRAs; 64: e31-e39, 2011.

3-Fitzpatrick RE, Rostan EF. Reversal of photodamage with topical growth factors: a pilot study. J Cosmet Laser Ther; 5(1): 25-34, 2003.

4-Man D, Plosker H, Winland-Brown JE. The use of autologous platelet rich plasma (platelet gel) and autologous platelet poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg; 107(1): 229-239, 2001.

5-Welsh WJ. Autologous platelet gel-clinical function and usage in plastic surgery. Cosmetic Derm ; 13:13–18, 2000.

3-References on Growth Factors in Cosmetics:

1-Mehta RC, Fitzpatrick RE. Endogenous growth factors as cosmeceuticals. Dermatologic Therapy; Published online; DOI: 10.1111/j.1529-8019.2007.00149.x,2007.

2-Michael H. Gold MD, Mitchel P. Goldman MD, Julie Biron Efficacy of Novel Skin Cream Containing Mixture of Human Growth Factors and Cytokines for Skin Rejuvenation. Journal of Drugs in Dermatology; 6 (2):197-202, 2007.

3-Sundaram H, Mehta RC, Norine JA, Kircik L, Cook-Bolden FE et al. Topically applied physiologically balanced growth factors: a new paradigm of skin rejuvenation. J. Drugs Dermatol ; 8(5 Suppl Skin Rejuvenation):4-13, 2009.

4-References on Growth Factors in Alopecia:

1-Bernstein RM et al. The aesthetics of follicular transplantation. Dermatol Surg; 23:785-799, 1997.

2-Bernstein RM et al. The art of repair in surgical hair restoration part 1: basic repair strategies.Dermatol Surg; 28: 783-794, 2002.

3-Du Toit DF et al. Soft and hard-tissue augmentation with platelet-rich plasma: Tissue culture dynamics, regeneration and molecular biology perspective. International Journal of Shoulder Surgery; 1: 64-73, 2007.

4-Du Toit DF et al. Shoulder surgeon and autologous cellular regeneration (ACR)-from bench to bed; Part 1-the link between the human fibroblast, connective tissue disorders and shoulder. International Journal of Shoulder Surgery; 1, 3 : 87-95, 2007.

5-Eppley BL, Pietrzak WS, Blanton W. Platelet-rich plasma: A review of biology and applications in plastic surgery. Plast Reconstr Surg; 18:147E-159C, 2006.

6-Ebstein JS. Revision surgical hair restoration: repair of undesirable results. Plast Reconstruct Surg; 104: 222-232, 1999.

7-Greco JF et al. A “ crush study” review of micrograft survival. Dermatol Surg; 23:752-755, 1997.

8-Hwang S et al. Does the recipient site influence the hair growth characteristics in hair transplantation. Dermatol Surg; 28: 795-8, 2002.

9-Kierszenbaum AL. Histology and cell biology. An introduction to pathology, Mosby, St Louis ; 106-108, 2002.

10-Kumar V, Cotran RS, Robbins SL. Robbins Basic Pathology. 7th edit. , Saunders, Philadelphia ; 74-75, 2002.

11-Marx RE, Garg AK. Dental and Craniofacial applications of platelet-rich plasma. Quintessence books, Chicago; 1-154, 2005.

12- Mazzucco L et al. Platelet rich plasma and platelet gel preparation using Plateltex®; Vox Sanguinis; 1: 1-7, 2008.

13-Pietrzak WS, Eppley BL. Platelet-rich plasma. Biology and new technology. J. Craniofacial Surg; 16:1043-1054, 2005.

14-Pietrzak WS, Yuehuei H, Qian K, Harry AD, Ehrens CH. Platelet-Rich and Platelet poor plasma: Development of an animal model to evaluate hemostatic efficacy. Cranial Facial Surgery; 18 : 559-566, 2007.

5-References on Growth Factors in Dentistry:


1-Abernathy W et al. Nonmetallic Fixation in Elective Maxillofacial Surgery. AORN Journal; 71:193-198, 2000.

2-Anitua E. Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants. J Oral Implantol;14:529–535,1999.

3-Artzi ZC, Newmcovsky C. The Application of Deproteinized Bovine Bone Mineral for Ridge Preservation Prior to Implantation. Clinical and Histological Observations in a Case Report. Journal of Periodontal; 69:1062-1067, 1998.

4-Ashman A. Ridge Preservation – The Future Practice of Dentistry, Dental Economics; 80-83, 1995.

5-Bahat O et al. Preservation of Ridges Utilizing Hydroxyapatite, The International Journal of Periodontics and Restorative Dentistry; 35-41, 1987.

6-Bell D. Particles Versus Solid Forms of Hydroxyapatite As A Treatment Modality To Preserve Residual Alveolar Ridges, The Journal of Prosthetic Dentistry; 56:323-326, 1986.
7-Carlson NE, Roach Jr RB. Platelet-rich plasma: Clinical applications in dentistry. JADA; 133 (10):1383-1386, 2002.

8-Danesh-Meyer MJ, Filstein MR, Shanaman R. Histological evaluation of sinus augmentation using platelet rich plasma (PRP): a case series. J Int Acad Periodontol; 3(2):48-56, 2001.

9-Frourn SJ, Tarnow DP. Wallace SS, Rohner MD, Cho SC. Sinus Floor Elevation Using An organic Bovine Bone Matrix (OsteoGraf/N) with and Without Autogenous Bone: A clinical, Histologic, Radiographic, and Histomorphometric Analysis-Part 2 of an Ongoing Prospective Study. The International Journal of Periodontics & Restorative Dentistry; 18:529-544, 1998.

10-Frourn SJ, Wallace SS, Tarnow DP, Cho SC. Effect of platelet rich plasma on bone growth and osseointegration in human maxillary sinus grafts: three bilateral case reports. Int J Periodont Restor Dent;22(1):45-53, 2002.

11-Garg AK. Chapter 5 - Grafting Materials in Repair and Restoration, Tissue Engineering – Applications in Maxillofacial Surgery and Periodontics, 83-101. Garg AK et al. Using Platelet-Rich Plasma to Develop Bone Grafts, Dental Implants and Periodontics. Dental Implantology Update; 11: 41-44, 2000.

12-Garg AK et al. The Use of Platelet-Rich Plasma to Enhance the Success of Bone Grafts Around Dental Implants. Dental Implantology Update; 11:17-20, .2000.

13-Garg AK et al. Using Platelet-Rich Plasma to Develop an Autologous Membrane for Growth Factor Delivery in Dental Implant Therapy. Dental Implantology Update; 11:41-44, 2000.

14-Garg AK. Lateral Proximal Tibia Bone Harvest for Use in Augmentation Procedures. Dental Impantology Update; 11(5): 33-40, 2001.

15-Garg AK. Bone Induction With and Without Membranes and Using Platelet-Rich Plasma. Oral and Maxillofacial Clinics of North America; 13 (3):437-448, 2001.

16-Graziani F et al. In vitro effects of different concentration of PRP on primary bone and gingival cell lines. Preliminary results. Minerva Stomatol; 54:15-22, 2005.

17-Gross J. Ridge Preservation Using HTR Synthetic Bone Following Tooth Extraction. General Dentistry; 43 (4): 364-367,1995.

18-Hoen M et al. Preserving the Maxillary Anterior Alveolar Ridge Contour Using Hydroxyapatite. JADA; 118:739-741, 1989.

19-Irwin C. Polypeptide growth factors-a role in restorative dentistry? Eur J Prosthodont Restor Dent;3(5):227-30, 1995.

20-Kaigler D, Avila G, Wisner-Lynch L, Nevins ML, Nevins M, Rasperini G, Lynch SE, Giannobile WV. Platelet-derived growth factor applications in periodontal and peri-implant bone regeneration. Expert Opin Biol Ther; 11(3):375-85, 2011.

21-Kassolis JD, Rosen PS, Reynolds MA. Alveolar ridge and sinus augmentation utilizing platelet-rich plasma in combination with freeze-dried bone allograft: case series. JPeriodontol;71:1654–1661,2000.

22-Kim SG, Chung CH, Kim YK, Park JC, Lim SC. Use of particulate dentin-plaster of Paris combination with/without platelet rich plasma in the treatment of bone defects around implants. Int J Oral Maxillofac Implant ; 17(1):86-94, 2002

22-Landesberg R et al. Quantification of Growth Factor Levels Using a Simplified Method of Platelet-Rich Plasma Gel Preparation. Journal of Oral & Maxillofacial Surgery; 58:297-300, 2000.

23-Landesberg R et al. Risks of Using Platelet Rich Plasma Gel. Journal of Oral Maxiofacial Surgery; 56:1116-1117, 1998.

24-Lekovic V et al. Preservation of Alveolar Bone in Extraction Sockets Using Bioabsorbable Membranes. Journal of Periodontal; 69:1044-1049, 1998.

25-Loo WD. Ridge preservation with immediate treatment dentures. Immediate dentures for Ridge preservation; 5-11, 1968.

26-Lozada JL, Caplanis N, Proussaefs P, Kammeyer G. Platelet-Rich Plasma Application in Sinus Graft Surgery: Part I-Background and Processing Techniques. Clinical; 38-42, 2001.

27-Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE and Georgeff KR: Platelet-rich plasma, growth factor enhancement for bone grafts. Oral Surgery Oral Medicine Oral Pathol; 85: 638-646, 1998.

28-Marx RE, Garg AK. Bone Structure, Metabolism, and Physiology: Its Impact on Dental Implantology. Implant Dentistry; 7:267-276, 1998.

29-Marx RE. Platelet-Rich Plasma: A Source of Multiple Autologous Growth Factors for Bone Grafts, In: Lynch SE, Genco RJ, Marx RE (eds). Tissue Engineering: Applications in Maxillofacial Surgery and Periodontics.Chicago: Quintessence:71-82,1999.

30-Marx RE. Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dent ;10:225–228,2001.

31-Marx RE. Platelet-rich plasma. Evidence to support its use. J Oral Maxillofac Surg; 62:489-496, 2004.

32-Misch CM, Misch CE. Chapter 31 – Intraoral Autogenous Donor Bone Grafts for Implant Dentistry. Contemporary Implant Dentistry. 2nd ed., 497-508.

33-Murray CG, Adkins KF.The elective retention of vital roots for alveolar bone preservation a pilot study. J. Oral Surgery; 650-656, 1979.

34-Nemcovsky CE, Serfaty V. Alveolar Ridge Preservation Following Extraction of Maxillary Anterior Teeth. Report on 23 Consecutive Cases. Journal of Periodontal; 67:390-395, 1996.

35- Nikolidakis D, Jansen JA. The biology of platelet-rich plasma and its application in oral surgery: literature review. Tissue Eng Part B ;14(3):249-58, 2008.

36-Okuda K et al .Platelet-rich plasma contains high level of platelet-derived growth factors and transforming growth factor beta and modulates the proliferation of periodontally related cells in vitro. J Periodontol; 74: 849-857, 2003.

37-Peleg M, Mazor Z, Garg AK. Augmentation Grafting of the Maxillary Sinus and Simultaneous Implant Placement in Patients with 3 to 5 mm of Residual Alveolar Bone Height. The International Journal of Oral & Maxillofacial Implants; 145-156, 1999.

38-Petrungaro P. The Use of Platelet Rich Plasma with Growth Factor (Autologous Platelet Gel) to Enhance Hard and Soft Tissue Healing and Maturation in Surgical Implant Dentistry. Contemporary Periodontics and Implantology; 1 (8): 2000.

39-Petrunagaro P.The Use of Platelet Rich Plasma with Growth Factors (Autologous Platelet Gel) to Enhance Hard and Soft Tissue Healing and Maturation in the Reconstruction of the Maxillary Pneumatized Sinus. Contemporary Periodontics and Implantology; 1 (9): February-March 2001.

40-Petrungaro PS. Using platelet-rich plasma to accelerate soft tissue maturation in esthetic periodontal surgery. Compend Contin Educ Dent ;22(9):729–736,2001.

41-Petrungaro P.Immediate Restoration of Dental Implants in the Aesthetic Zone, Dental Implantology Update; 12 (12), December 2001.

42-Rodriquez A, Anastassov G, Lee H, Buchbinder D, Wettan H.Maxillary sinus Augmentation With Deproteinated Bovine Bone and Platelet Rich Plasma With simultaneous Insertion of Endosseous Implants. American Association of Oral and Maxillofacial Surgery;61:157-163,2003.

43-Petrungaro P. Platelet-Rich Plasma for Dental Implants and Soft-Tissue Grafting. Dental Implantology Update; 11 (6): 41-46, 2001.

44-Quinn J et al. Preservation of the Alveolar Ridge With Hydroxyapatite Tooth Root Substitutes. JADA; 110:189-193, 1985.

45-Quinn J, Kent J.Alveolar Ridge Maintenance With Solid Nonporous Hydroxyapatite, The International Journal of Periodontics and Restorative Dentistry ; 35-41, 1987.

46-Raghoebar GM, Schortinghuis J, Liem RS, Ruben JL, van der Wal JE, Vissink A. Does platelet-rich plasma promote remodeling of autologous bone grafts used for augmentation of the maxillary sinus floor? Clin Oral Implants Res; 16:349-356, 2005.

47-Robiony M, Polini F, Costa F, Pasca M and Politi M: Vertical augmentation in severe atrophic mandible: osteogenesis distraction and platelet-rich plasma (PRP). Rivista Italiana Chirurgia Maxillo-Facciale; 12: 3-7, 2001.

48-Robiony M, Polini F, Costa F, et al. Osteogenesis distraction and platelet-rich plasma for bone restoration of the severely atrophic mandible: preliminary results. J Oral Maxillofac Surg ;60:630–635,2002.

49-Rosenberg ES, Torosion J. Sinus Grafting Using Platelet-Rich Plasma – Initial Case Presentation. Pract Periodont Aesthet Dent; 12(9): 843-850, 2000.

50-Scala M, Gipponi M, Margarino G, Mereu P, Orsi A and Strada P: Platelet concentrate in maxillofacial surgery. Proc American Academy Maxillofacial Prosthetics. International Congress Maxillofacial Prosthetics, Maxillofacial Prosthetics in the 21st Century, Kauai, Hawaii, pp. 132, 2000.

51-Scala M, Gipponi M, Pasetti S, Dellachá E, Ligorio M, Villa G, Margarino G, Giannini G, Strada P. Clinical applications of autologous cryoplatelet gel for the reconstruction of the maxillary sinus. A new approach for the treatment of chronic oro-sinusal fistula. In Vivo; 21(3):541-547, 2007.

52-Sclar A.Preserving Alveolar Ridge Anatomy Following Tooth Removal In Conjunction With Immediate Implant Placement – The Bio-Col Technique. Atlas of the Oral and Maxillofacial Surgery Clinics of North America; 7:39-59, 1999.

53-Sclar A.Use Of The Epithelialized Palatal Graft With Dental Implants. Atlas of the Oral and Maxillofacial Surgery Clinics of North America; 7:79-94, 1999.

54-Seibert JS, Salama H. Alveolar Ridge Preservation and Reconstruction. Periodontology 2000;11:69-84, 1996.

55. Shashikiran ND, Reddy VV,Yavagal CM, Zakirulla M. Applications of platelet-rich plasma (PRP) in contemporary pediatric dentistry. J Clin Pediatr Dent ; 30(4):283-6, 2006.

56-Shanaman R, Filstein MR, Danesh-Meyer MJ. Localized ridge augmentation using GBR and platelet-rich plasma: case reports.Int J Periodontics Restorative Dent; 21:345–355,2001.

57-Soffer E, Ouhayoun JP, Anagnostou F. Fibrin sealants and platelet preparations in bone and periodontal healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod ; 95:521–528,2003.

58-Sonnleitner D, Huemer P, Sullivan DY. A Simplified Technique for Producing Platelet-Rich Plasma and Platelet Concentrate for Intraoral Bone Grafting Techniques: A Technical Note. International Journal of Oral & Maxillofacial Implants; 15:879-882, 2000.

59-Stefani CM, Machado MAN.Platelet-Derived Growth Factor/Insulin-Like Growth Factor-1 Combination and Bone Regeneration Around Implants Placed Into Extraction Sockets: A Histometric Study in Dogs. Implant Dentistry; 126-130, 2000.

60-Tyler SA.Office-Based Harvest of Platelet-Rich Plasma for Intraoral Bone Grafting, Poster 34, AAOMS, 2000.

61-Whitman DH et al. A Technique for Improving the Handling of Particulate Cancellous Bone and Marrow Grafts Using Platelet Gel. Journal of Oral Maxiofacial Surgery,; 45:1217-1218,198.

62-Whitman DH et al.Platelet Gel: An Autologous Alternative to Fibrin Glue with Applications in Oral and Maxillofacial Surgery. Journal Oral Maxillofacial Surgery; 55:1294-1299, 1997.

25-Whitman DH, Berry RL and Green DM. Platelet gel: an alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillo-Facial Surg; 55: 1294-1299, 1997.

6-References on Growth Factors in Wound healing and Orthopedic Surgery:

6.1 - Humans:

1-Adler SC, Kent KJ. Enhancing healing with growth factors. Facial Plast Surg Clin North Am;10(2):129-146, 2002.

2-Alsousou J, Thompson M, Hulley P, Noble A, Willet K. The biology of platelet-rich plasma and its application in trauma and orthopaedic surgery: a review of the literature. J Bone Joint Surg Br ; 91(8):987-996,2009.

3-Anitua E, Andia I, Ardanza B, Nurden P, Nurden AT. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost ; 91(1):4-15, 2004.

4-Anitua E, Andia I, Sánchez M, Azofra J, del Mar Zalduendo M, de la Fuente M, Nurden P, Nurden AT.Autologous preparations rich in growth factors promote proliferation and induce VEGF and HGF production by human tendon cells in culture.J Orthop Res ; 23:281–286,2005.

5-Anitua E, Sánchez M, Nurden AT, et al. Platelet-released growth factors enhance the secretion of hyaluronic acid and induce hepatocyte growth factor production by synovial fibroblasts from arthritic patients. Rheumatology (Oxford); 46(12):1769-1772, 2007.

6-Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andia I.New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 24:227–234, 2006.

7-Anitua E, Sanchez M, Nurden AT, Zalduendo M, de la Fuente M, Azofra J, Andia I. Reciprocal actions of platelet secreted TGF-beta1 on the production of VEGF and HGF by human tendon cells. Plast Reconstr Surg 119:950–959,2007.

8-Arpornmaeklong P, Kochel M, Depprich R, Kübler NR, Würzler KK. Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study. Int J Oral Maxillofac Surg 33:60–70,2004.

9-Aspenberg P. Stimulation of tendon repair: mechanical loading, GDFs and platelets. A mini-review. Int Orthop 31:783–789,2007.

10-Aspenberg P, Virchenko O. Platelet concentrate injection improves Achilles tendon repair in rats. Acta Orthop Scand 75:93–99,2004.

11-Barrientos s, Stojanovic O, Golinko MS, Brem H and Tomic-Canic M. Growth factors and cytokines in wound healing. Wound Repair Regen ; 16: 585-601,2008.

12-Barrett S, Erredge S. Growth factors for chronic plantar fascitis.Podiatry Today; 7:37-42, 2004.

13-Bennett NT, Schultz GS. Growth factors and wound healing: biochemical
properties of growth factors and their receptors. Am JSurg ;165(6):728-737,1993.

14-Bennett NT and Schultz GS: Growth factors and wound healing: Part II. Role in normal and chronic wound healing. Am J Surg; 166: 74-81, 1993.

15-Berghoff W, Pietrzak W, Rhodes R. Platelet-rich plasma application during closure following total knee arthroplasty. Orthopedics ;29(7):590-598, 2006.

16-Crovetti G, Martinelli G, Issi M, et al. Platelet gel for healing cutaneous chronic wounds. Transfus Apher Sci ; 30(2):145-151, 2004.

17-Cullinane AB, O’Callaghan P, McDermott K, Keohane C, Cleary PE. Effects of autologous platelet concentrate and serum on retinal wound healing in an animal model. Graefe’s Arch Clin Exp Ophthalmol ; 240(1):35-41, 2002.

18-Dallari D, Savarino L, Stagni C, et al. Enhanced tibial osteotomy healing with use of bone grafts supplemented with platelet gel or platelet gel and bone marrow stromal cells. J Bone Joint Surg Am ;89(11):2413-2420, 2007.

19-Delrossi AJ, Cernaianu AC, Vertrees RA, et al. Platelet-rich plasma reduces postoperative blood loss. J Thorac Cardiovasc Surg;100(2):281-286, 2000.

20-De Mos M, van der Windt AE, Jahr H, van Schie HT, Weinans H, Verhaar JA, van Osch GJ. Can platelet-rich plasma enhance tendon repair? A cell culture study. Am J Sports Med; 36(6):1171-1178, 2008.

21-de Vos RJ, Weir A, van Schie HT, et al. Platelet-rich plasma injection for chronic Achilles tendinopathy: a randomized controlled trial. JAMA ; 303(2):144-149, 2010.

22-Driver VR, Hanft J, Fylling CP, Beriou JM. Autologel Diabetic Foot Ulcer Study Group. A prospective, randomized, controlled trial of autologous platelet-rich plasma gel for the treatment of diabetic foot ulcers. Ostomy Wound Manage ; 52(6):68-70,2006.

23-Edwards S, Calandruccio J. Autologous blood injections for refractory lateral epicondylitis. J Hand Surg Am ; 28(2):272-278, 2003.


24-Eppley BL, Woodell JE, Higgins J. Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. Plast Reconstr Surg; 114(6):1502-1508, 2006.

25-Everts PA, Devilee RJ, Brown Mahoney C, et al. Platelet gel and fibrin sealant reduce allogenic blood transfusions in total knee arthroplasty. Acta Anaesthesiol Scand ; 50(5):593-599, 2006.

26-Frechette JP, Martineau I, Gagnon G. Platelet rich plasmas: growth factor content and roles in wound healing. J Dent Res ; 84(5):434-439, 2005.

27-Fufa D, Shealy B, Jacobson M, Kevy S, Murray MM. Activation of platelet-rich plasma using soluble type I collagen. J Oral Maxillofac Surg ;66(4):684-690, 2008.

28-Gamradt SC, Rodeo SA, Warren RF. Platelet rich plasma in rotator cuff repair. Tech Orthop ; 22:26-33, 2007.

29-Gandhi A, Doumas C, O’Connor JP, Parsons JR, Lin SS. The effects of local platelet rich plasma delivery on diabetic fracture healing. Bone ; 38:540-546, 2006.

30-Gardner MJ, Demetrakopoulos D, Klepchick P, Mooar P. The efficacy of autologous platelet gel in pain control and blood loss in TKR: an analysis of the haemoglobin, narcotic requirement and range of motion. Int Orthop; 31:309-313,2007.

31-Han B, Woodell-May J, Ponticiello M, Yang Z, Nimni M. The effect of thrombin activation of platelet-rich plasma on demineralized bone matrix osteoconductivity. J Bone Joint Surg Am ;91(6): 1459-1470, 2009.

32-Ganio C, Tenewitz FE, Wilson RC, Moyles BG. The treatment of chronic nonhealing wounds using autologous platelet-derived growth factors. J Foot Ankle Surg ; 32(3):263-8, 1993.

33-Govindarajan B, Shah A, Cohen C, et al.Malignant transformation of human cells by constitutive expression of platelet-derived growth factor-BB. J Biol Chem; 280 (14):13936-13943.

34-Greenhalgh DG: The role of growth factors in wound healing. J Trauma Injury Infection Critical Care ; 41: 159-167, 1996.

35-Hammond JW, Hinton RY, Curl LA, Muriel JM, Lovering RM. Use of autologous platelet-rich plasma to treat muscle strain injuries. Am J Sports Med ; 37(6):1135- 1142, 2009.

36-Henderson JL, Cupp CL, Ross EV, et al. The effects of autologous platelet gel on wound healing. Ear Nose Throat J ; 82(8):598-602, 2003.

37-Hiramatsu T, Okamura T, Imai Y, et al. Effects of autologous platelet concentrate reinfusion after open heart surgery in patients with congenital heart disease. Ann Thorac Surg ; 73(4):1282–1285, 2002.

38-Kajikawa Y, Morihara T, Sakamoto H, Matsuda K, Oshima Y, Yoshida A, Nagae M, Arai Y, Kawata M, Kubo T. Platelet-rich plasma enhances the initial mobilization of circulation-derived cells for tendon healing. J Cell Physiol; 215(3):837-845, 2008.

39-Kasemkijwattana C, Menetrey J, Bosch P, et al. Use of growth factors to improve muscle healing after strain injury. Clin Orthop Relat Res ; 370:272-285, 2000.

40-Kanno T, Takahashi T, Tsujisawa T, Ariyoshi W, Nishihara T. Platelet-rich plasma enhances human osteoblast-like cell proliferation and differentiation. J Oral Maxillofac Surg ; 63(3):362-369, 2005.

41-Kazakos K, Lyras DN, Verettas D, Tilkeridis K, Tryfonidis M. The use of autologous PRP gel as an aid in the management of acute trauma wounds. Injury; 40(8):801-805, 2009.

42-Kitoh H, Kitakoji T, Tsuchiya H, Katoh M, Ishiguro N. Transplantation of culture expanded bone marrow cells and platelet rich plasma in distraction osteogenesis of the long bones. Bone;40(2):522-528, 2007.

43-Knighton DR, Ciresi KF, Fiegel VD, Austin LL, Butler EL. Classification and treatment of chronic nonhealing wounds. Successful treatment with autologous platelet-derived wound healing factors (PDWHF). Ann Surg ; 204(3):322-330, 1986.

44-Knighton DR, DoucetteM, Fiegel VD, Ciresi K, Butler E, Austin L. The use of platelet derived wound healing formula in human clinicaltrials. Prog Clin Biol Res ; 266:319-329, 1988.

45-Kon E, Filardo G, Delcogliano M, et al. Platelet-rich plasma: new clinical application. A pilot study for treatment of jumper’s knee. Injury ; 40(6):598-603,2009.

46-Krupski WC, Reilly LM, Perez S, Moss KM, Crombleholme PA, Rapp JH. A prospective randomized trial of autologous platelet-derived wound healing factors for treatment of chronic nonhealing wounds: a preliminary report. J Vasc Surg; 14(4):526-532, 1991.

47-Kunimoto BT. Growth factors in wound healing:the next great innovation? Ostomy Wound Manage ; 45(8):56-64, 1999.

48-Lindeboom JA, Mathura KR, Aartman IH, Kroon FH, Milstein DM, Ince C. Influence of the application of platelet-enriched plasma in oral mucosal wound healing. Clin Oral Implants Res ; 18(1):133-139, 2007.

49-Lynch SE, Nixon JC, Colvin RB, Antoniades HN. Role of platelet-derived growth factor in wound healing: synergistic effects with other growth factors. Proc Natl Acad Sci USA; 84(21):7696-7700, 1987.

50-Lynch SE, Colvin RB, Antoniades HN. Growth factors in wound healing. Single and synergistic effects on partial thickness porcine skin wounds. J Clin Invest ; 84: 640– 646, 1989.

51-Margolis DJ, Kantor J, Santanna J, Strom BL, Berlin JA. Effectiveness of platelet releasate for the treatment of diabetic neuropathic foot ulcers. Diabetes Care; 24(3):483-8, 2001.

52-Margolis DJ, Bartus C, Hoffstad O, Malay S, Berlin JA. Effectiveness of recombinant human platelet-derived growth factor for the treatment of diabetic neuropathic foot ulcers. Wound Repair Regen ; 13(6):531-536, 2005.

53-Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod ; 85(6):638-646, 1998.

54- Marx RE. Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dent ; 10(4):225-228, 2001.

55-Marx RE. Platelet-rich plasma: evidence to support its use. J O Growth Factors; 24(3):165-171, 2006.

56-McAleer JP, Sharma S, Kaplan EM, Persich G. Use of autologous platelet concentrate in a nonhealing lower extremity wound. Adv Skin Wound Care; 9(7):354-363, 2006.

57-Mehta S, Watson JT. Platelet rich concentrate: basic science and current clinical applications. J Orthop Trauma ; 22(6):432-438. 2008.

58-Menetrey J, Kasemkijwattana C, Day CS, et al. Growth factors improve muscle healing in vivo. J Bone Joint Surg Br. 2000;82(1):131-137.

59-Mishra A, Pavelko T. Treatment of chronic elbow tendinosis with buffered platelet-rich plasma.Am J Sports Med; 34(11):1774-1778, 2006.

60-Mishra A, Woodall J Jr, Vieira A.Treatment of Tendon and Muscle using platelet-rich plasma, Clin Sports Med; 28(1):113-125,2009.

61-Mishra A, Woodall J Jr, Vieira A. Treatment of tendon and muscle using platelet-rich plasma. Clin Sports Med ; 28(1):113-125, 2009.

62-Molloy T, Wang Y, Murrell G. The roles of growth factors in tendon and ligament healing. Sports Med ;33(5):381-394, 2003.

63-Nagumo A, Yasuda K, Numazaki H, et al. Effects of separate application of three growth factors (TGF-beta 1, EGF, and PDGF-BB) on mechanical properties of the in situ frozen-thawed anterior cruciate ligament. Clin Biomech ; 20(3):283-290, 2005.

64-Nurden AT,Nurden P, Sanchez M, Andia I and Anitua E. Platelets and wound healing.Front Biosci; 13:3532-3548,2008.

65-O’Connell SM, Impeduglia T, Hessler K, Wang XJ, Carroll RJ, Dardik H. Autologous platelet-rich fibrin matrix as cell therapy in the healing of chronic lower-extremity ulcers. Wound Repair Regen; 16(6):749-756, 2008.

66-Orrego M, Larrain C, Rosales J, et al. Effects of platelet concentrate and a bone plug on the healing of hamstring tendon in a bone tunnel. Arthroscopy ; 24(12):1373-1380, 2008.

67-Pietramaggiori G, Kaipainen A, Czeczuga JM, Wagner CT, Orgill DP. Freeze-dried platelet-rich plasma shows beneficial healing properties in chronic wounds.Wound Repair Regen ; 14(5):573-580, 2006.

68-Sampson S, Gerhardt M, Mandelbaum B. Platelet rich plasma injection grafts for musculoskeletal injuries: a review. Curr Rev Musculoskeletal Med ; 1(3-4):165-174, 2008.

69-Randelli PS, Arrigoni P, Cabitza P, Volpi P, Maffulli N. Autologous platelet rich plasma for arthroscopic rotator cuff repair: a pilot study. Disabil Rehabil; 30(20-22):1584-1589, 2008.

70-Reboa G, Gipponi M, Testa T, Giannini G, Scala M, Dalla Costa R, Strada P. Regenerative medicine for the definitive surgical repair of pilonidal sinus. A new method of wound reconstruction. In Vivo; 21(3):529-534, 2007.

71-Robson MC, Phillips LG, Thomason A, et al. Recombinant human platelet-derived growth factor-BB for the treatment of chronic pressure ulcers. Ann Plast Surg ; 29(3):193-201, 1992.

72-Roukis TS et al. Autologous platelet-rich plasma for wound and osseous healing. A review. Adv ther; 23: 218-237, 2006.

73-Rozman P, Bolta Z. Use of platelet growth factors in treating wounds and soft-tissue injuries. Acta Dermatovenerol Alp Panonica Adriat;16(4):156-165, 2007.

74-Salemi S, Rinaldi C, Manna F, Guarneri GF, Parodi PC. Reconstruction of lower leg skin ulcer with autologous adipose tissue and platelet-rich plasma. J Plast Reconstr Aesthet Surg ; 61(12):1565-1567, 2008.

75-Sánchez M, Anitua E, Azofra J, Andía I, Padilla S, Mujika I. Comparison of surgically repaired Achilles tendon tears using platelet-rich fibrin matrices. Am J Sports Med; 35(2):245-251, 2007.

76-Sánchez M, Anitua E, Cugat R, et al. Nonunions treated with autologous preparation rich in growth factors. J Orthop Trauma ; 23(1):52-59, 2009.

77-Savarino L, Cenni E, Tarabusi C, et al. Evaluation of bone healing enhancement by lyophilized bone grafts supplemented with platelet gel: a standardized methodology in patients with tibial osteotomy for genu varus. J Biomed Mater Res B Appl Biomater; 76(2):364-372, 2006.

78-Schmidt MB, Chen EH, Lynch SE. A review of the effects of insulin-like growth factor and platelet derived growth factor on in vivo cartilage healing and repair. Osteoarthritis Cartilage ; 14(5):403-412, 2006.

79-Schnabel LV, Mohammed HO, Miller BJ, et al. Platelet rich plasma (PRP) enhances anabolic gene expression patterns in flexor digitorum superficialis tendons. J Orthop Res; 25(2):230-240, 2007

80-Shah M, Revis D, Herrick S, Baillie R, Thorgeirson S, Ferguson M, and Roberts A. Role of elevated plasma transforming growth factor- beta1 levels in wound healing. Am J Pathol ; 154: 1115–1124, 1999.

81-Shimo T, Nakanishi T, Nishida T, Asano M, Kanyama M, Kuboki T, Tamatani T, Tezuka K, Takemura M, Matsumara T, Takigawa M. Connective tissue growth factor induces the proliferation, migration, and tube formation of vascular endothelial cells in vitro, and angiogenesis in vivo. J Biochem ; 126: 137–145, 1999.

82-Silva A, Sampaio R. Anatomic ACL reconstruction: does the platelet-rich plasma accelerate tendon healing? Knee Surg Sports Traumatol Arthrosc ; 17(6):676-682, 2009.

83-Steed DL, Goslen JB, Holloway GA, Malone JM, Bunt TJ, Webster MW. Randomized prospective double-blind trial in healing chronic diabetic foot ulcers. CT-102 activated platelet supernatant, topical versus placebo. Diabetes Care ; 15(11):1598-1604, 1992.

84-Steed DL. Clinical evaluation of recombinant human platelet-derived growth factor for the treatment of lower extremity diabetic ulcers. Diabetic Ulcer Study Group. J Vasc Surg; 21(1):71-78, 1995.

85-Steed DL. Modifying the wound healing response with exogenous growth factors. Clin Plast Surg ; 25: 397–405, 1998.

86-Virchenko O, Aspenberg P. How can one platelet injection after tendon injury lead to a stronger tendon after 4 weeks? Interplay between early regeneration and mechanical stimulation. Acta Orthop ; 77(5):806-812, 2006.

87-Vogt PM, Lenhardt M, Wagner D, Jansen V, Krieg M, Steinau HU. Determination of endogenous growth factors in human wound fluid: temporal presence and profiles of secretion. Plast Reconstr Surg ; 102: 117–123, 1998.

88-Werner S. Keratinocyte growth factor: a unique player in epithelial repair processes.Cytokine Growth Factor ; Rev 2: 153–165, 1998.

89-Woodall J Jr, Tucci M, Mishra A, Benghuzzi H. Cellular effects of platelet rich plasma: a study on HL-60 macrophage-like cells, Biomed Sci Instrum; 43:266-271, 2007.

90-Wrotniak M, Bielecki T, Gazdzik TS. Current opinion about using the platelet-rich gel in orthopaedics and trauma surgery. Ortop Traumatol Rehabil ; 9(3):227-238, 2007.

91-Wu W, Zhang J, Dong Q, Liu Y, Mao T, Chen F. Platelet-rich plasma: a promising cell carrier for micro-invasive articular cartilage repair. Med Hypotheses ; 72(4):455-457, 2009.

92-Yamada Y, Ueda M, Naiki T, Takahashi M, Hata K, Nagasaka T. Autogenous injectable bone for regeneration with mesenchymal stem cells and platelet-rich plasma: tissue-engineered bone regeneration. Tissue Eng; 10:955-964, 2004.

93-Yu W, Naim JO, Lanzafame RJ. Expression of growth factors in early wound healing in rat skin. Lasers Surg Med ; 15: 281–289, 1994.

6.2 - Animals (Horses):

1-Bosch G, Moleman M, Barneveld A, van Weeren PR, van Schie HT. The effect of platelet-rich plasma on the neovascularization of surgically created equine superficial digital flexor tendon lesions. Scand J Med Sci Sports ; 21(4):554-561, 2011.

2-Bosch G, René van Weeren P, Barneveld A, van Schie HT. Computerised analysis of standardised ultrasonographic images to monitor the repair of surgically created core lesions in equine superficial digital flexor tendons following treatment with intratendinous platelet rich plasma or placebo. Vet J ; 187(1):92-98, 2011.

3-Bosch G, van Schie HT, de Groot MW, Cadby JA, van de Lest CH, Barneveld A, van Weeren PR. Effects of platelet-rich plasma on the quality of repair of mechanically induced core lesions in equine superficial digital flexor tendons: A placebo-controlled experimental study. J Orthop Res ; 28(2):211-217, 2010.

4-Carter CA, Jolly DG, Worden CE Sr., et al. Platelet-rich plasma gel promotes differentiation and regeneration during equine wound healing. Exp Mol Pathol; 74:244–255,2003.
5-Del Bue M, Riccò S, Ramoni R, Conti V, Gnudi G, Grolli S. Equine adipose-tissue derived mesenchymal stem cells and platelet concentrates: their association in vitro and in vivo. Vet Res Commun ; 32 Suppl 1:S : 51-55, 2008.

6-DeRossi R, Coelho AC, Mello GS, Frazílio FO, Leal CR, Facco GG, Brum KB. Effects of platelet-rich plasma gel on skin healing in surgical wound in horses.
Acta Cir Bras ; 24(4):276-281, 2009.

7-Fennis JPM, Stoelinga PJW, Jansen JA. Mandibular reconstruction:a clinical and radiographic animal study on the use of autogenous scaffolds and platelet-rich plasma. J Oral Maxillofac Surg;31:281–286,2002.

8-Geburek F, Stadler P. [Regenerative therapy for tendon and ligament disorders in horses. Terminology, production, biologic potential and in vitro effects]. Tierarztl Prax Ausg G Grosstiere Nutztiere ; 39(6):373-383. 2011. German

9-Torricelli P, Fini M, Filardo G, Tschon M, Pischedda M, Pacorini A, Kon E, Giardino R. Regenerative medicine for the treatment of musculoskeletal overuse
injuries in competition horses. Int Orthop ; 35(10):1569-1576, 2011.

10-Textor JA, Norris JW, Tablin F. Effects of preparation method, shear force, and exposure to collagen on release of growth factors from equine platelet-rich
plasma. Am J Vet Res ; 72(2):271-278, 2011.

11-McCarrel T, Fortier L. Temporal growth factor release from platelet-rich plasma, trehalose lyophilized platelets, and bone marrow aspirate and their effect on tendon and ligament gene expression. J Orthop Res ; 27(8):1033-1042, 2009.

12-Schnabel LV, Mohammed HO, Miller BJ, McDermott WG, Jacobson MS, Santangelo KS, Fortier LA. Platelet rich plasma (PRP) enhances anabolic gene expression patterns in flexor digitorum superficialis tendons. J Orthop Res ; 25(2):230-240,2007.

13-Schnabel LV, Sonea HO, Jacobson MS, Fortier LA. Effects of platelet rich plasma and acellular bone marrow on gene expression patterns and DNA content of
equine suspensory ligament explant cultures. Equine Vet J ; 40(3):260-265, 2008.

14-Waselau M, Sutter WW, Genovese RL, Bertone AL. Intralesional injection of platelet-rich plasma followed by controlled exercise for treatment of midbody
suspensory ligament desmitis in Standardbred racehorses. J Am Vet Med Assoc 15;232(10):1515-1520, 2008.

15-Zhang YZ, Da WM. [Experimental study of platelet-rich plasma optimizing mesenchymal stem cells culture]. Zhonghua Xue Ye Xue Za Zhi (Chinese Journal of Hematology); 27(6):403-405, 2006. Chinese.

16-Smith JJ, Ross MW, Smith RK. Anabolic effects of acellular bone marrow, platelet rich plasma, and serum on equine suspensory ligament fibroblasts in
vitro. Vet Comp Orthop Traumatol; 19(1):43-47, 2006.